Hepatitis B surface antigen detection using pooled sera: A cost-benefit analysis
نویسندگان
چکیده
منابع مشابه
EXPRESSION OF HEPATITIS B SURFACE ANTIGEN IN SACCHAROMYCES CEREVISIAE
The genome of HB V virus of serotype ayw cloned in pBR322 and expression shuttle vector p YES2 were used for construction of the HBsAg chimeric genes and their expression in Saccharomyces cerevisiae. Two recombinant plasmids were constructed. One of them contained the coding sequences for the major polypeptide of surface antigen. Another construct carried the major polypeptide with the pre-S2 a...
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Routine use of the automated chemiluminescent microparticle immunoassay Abbott ARCHITECT anti-HBc for diagnosis of hepatitis B is limited in case of borderline reactive sera with low signal close to the cut-off index. In order to determine the significance of anti-HBc detection when borderline reactivity occurs using the ARCHITECT anti-HBc assay, a comparative study was designed. 3540 serum sam...
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AIMS To validate the sensitivity of universal antenatal screening for hepatitis B surface antigen (HBsAg) by testing pools of 10 sera, and to review 10 years' experience using this method. METHODS 66,945 antenatal patients were tested between 1986 and 1996 using the pooled method. All sera from 1996 (n = 6050) were retrieved and retrospectively tested individually. An in vitro determination o...
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A method has been developed for the analysis of hepatitis B surface antigen (HBsAg) antigenic structure at the molecular level that creates "fingerprints" or "signatures" of various hepatitis B viral (HBV) strains. This technique employs high affinity IgM and IgG monoclonal antibodies (anti-HBs) directed against distinct and separate determinants on HBsAg. In performing this antigenic structura...
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hepatitis b virus (hbv) infection is the 10th leading cause of death worldwide. the most important diagnostic and screening marker for hbv infection is hepatitis b surface antigen (hbsag), and the most widely used hbsag screening test is enzyme-linked immunosorbent assay (elisa). in this study, an elisa assay has been developed for detection of hbsag using two novel monoclonal antibodies (mab) ...
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ژورنال
عنوان ژورنال: Revista Española de Enfermedades Digestivas
سال: 2006
ISSN: 1130-0108
DOI: 10.4321/s1130-01082006000200006